General Rules and Regulations in the Microbiology Laboratory

 

General Rules and Regulations in the Microbiology Laboratory

1.     Always wear a clean laboratory coat to protect clothes and skin from spills and contamination.

2.    Disinfect the working bench with an appropriate disinfectant before starting any work and after finishing the work to maintain a clean and safe environment.

3.     Maintain an aseptic zone:

a.      Use a Bunsen burner to flame-sterilize inoculating loops, needles, and other tools before and after use.

b.     Work near a flame or laminar airflow to prevent contamination.

c.      Avoid unnecessary movement or talking during inoculation.

4.     Ensure all instruments and materials are sterile before use to avoid contamination of samples.

5.     Clearly label all samples, cultures, and chemicals to avoid mix-ups and ensure proper handling.

6.     Dispose of all biological waste in designated biohazard containers. Autoclave or otherwise properly sterilize waste before disposal.

7.     Tie long hair back and avoid touching your face, hair, or personal items during experiments

8.     Never pipette by mouth. Use mechanical pipetting devices.

9.     Do not eat, drink, or chew gum inside the laboratory.

10.  Dispose of all biological waste properly:

a.      Use designated containers for contaminated materials.

b.     Autoclave infectious waste before disposal.

11.  Report any spills or accidents to the instructor/lab supervisor immediately.

12.  Do not remove any materials (cultures, reagents, glassware) from the lab without permission.

13.  Wear gloves, face masks, or safety goggles if required for specific procedures involving hazardous organisms or chemicals.

14.  Turn off burners and electrical equipment after use.

15.  Follow proper handwashing and decontamination protocol even after wearing gloves.

16.  Be aware of the location of emergency exits, fire extinguishers, eye wash stations, and first aid kits.